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Comparing environmental DNA metabarcoding and underwater visual census to monitor tropical reef fishes ArchiMer
Polanco Fernández, Andrea; Marques, Virginie; Fopp, Fabian; Juhel, Jean‐baptiste; Borrero‐pérez, Giomar Helena; Cheutin, Marie‐charlotte; Dejean, Tony; González Corredor, Juan David; Acosta‐chaparro, Andrés; Hocdé, Régis; Eme, David; Maire, Eva; Spescha, Manuel; Valentini, Alice; Manel, Stéphanie; Mouillot, David; Albouy, Camille; Pellissier, Loïc.
Environmental DNA (eDNA) analysis is a revolutionary method to monitor marine biodiversity from animal DNA traces. Examining the capacity of eDNA to provide accurate biodiversity measures in species‐rich ecosystems such as coral reefs is a prerequisite for their application in long‐term monitoring. Here, we surveyed two Colombian tropical marine reefs, the island of Providencia and Gayraca Bay near Santa Marta, using eDNA and underwater visual census (UVC) methods. We collected a large quantity of surface water (30 L per filter) above the reefs and applied a metabarcoding protocol using three different primer sets targeting the 12S mitochondrial DNA, which are specific to the vertebrates Actinopterygii and Elasmobranchii. By assigning eDNA sequences to...
Tipo: Text Palavras-chave: Biodiversity; Biomonitoring; Caribbean Sea; Environmental DNA; Reef fishes; Underwater visual census.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00653/76543/77643.pdf
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Phylogenetic measures reveal eco‐evolutionary drivers of biodiversity along a depth gradient ArchiMer
Eme, David; Anderson, M. J.; Myers, E. M. V.; Roberts, C. D.; Liggins, L..
Energy and environmental stability are positively correlated with species richness along broad‐scale spatial gradients in terrestrial ecosystems, so their relative importance in generating and preserving diversity cannot be readily disentangled. This study seeks to exploit the negative correlation between energy and stability along the oceanic depth gradient to better understand their relative contribution in shaping broadscale biodiversity patterns. We develop a conceptual framework by simulating speciation and extinction along energy and stability gradients to generate expected patterns of biodiversity for a suite of complementary phylogenetic diversity metrics. Using a time‐calibrated molecular phylogeny for New Zealand marine ray‐finned fishes and a...
Tipo: Text Palavras-chave: Community phylogenetics; Deep sea; Energy gradient; Latitudinal gradient; Museum/cradle; New Zealand marine Actinopterygii.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00609/72071/70778.pdf
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